Journal: Materials

Article Title: Assessment of Titanate Nanolayers in Terms of Their Physicochemical and Biological Properties

doi: 10.3390/ma14040806

Figure Lengend Snippet: Viability of adipose-derived mesenchymal stem cells (ADSCs) cultured on the tested samples for 24, 72, and 120 h. The results are expressed as the mean ± SEM and presented as a percentage of the cells cultivated on the T samples (served as 100%). Asterisks indicate differences when the viability of cells growing on the tested scaffolds was higher in comparison with T samples (*** p < 0.001, ** p < 0.01, * p < 0.05). Pound signs denote differences between the cells cultured on the T5-S scaffolds and T-S samples (### p < 0.001).

Article Snippet: Adipose-derived human mesenchymal stem cells (ADSCs) were purchased from PromoCell and cultured in Mesenchymal Stem Cell Growth Medium ® supplemented with 10% Supplement Mix ® (PromoCell GmbH, Germany), 100 U/mL penicillin, and 100 µg/mL streptomycin.

Techniques: Derivative Assay, Cell Culture

Journal: Materials

Article Title: Assessment of Titanate Nanolayers in Terms of Their Physicochemical and Biological Properties

doi: 10.3390/ma14040806

Figure Lengend Snippet: Comparative SEM images showing human ADSCs cultured on the surface of T-S ( a ) and T5-S ( b ) specimens for 120 h. The figures confirm evident differences in cell morphology for both samples: adherent cells with filopodia attached to the surface of T-S ( a ) and rounded cells without attachments on T5-S ( b ).

Article Snippet: Adipose-derived human mesenchymal stem cells (ADSCs) were purchased from PromoCell and cultured in Mesenchymal Stem Cell Growth Medium ® supplemented with 10% Supplement Mix ® (PromoCell GmbH, Germany), 100 U/mL penicillin, and 100 µg/mL streptomycin.

Techniques: Cell Culture

Journal: ACS Biomaterials Science & Engineering

Article Title: Enhanced Vascular-like Network Formation of Encapsulated HUVECs and ADSCs Coculture in Growth Factors Conjugated GelMA Hydrogels

doi: 10.1021/acsbiomaterials.4c00465

Figure Lengend Snippet: Encapsulated HUVECs and hADSCs in 3D hydrogels. (A) Day 14 LIVE/DEAD imaging of encapsulated cells. The images are displayed in Z-projection and merged channels (Green: live cells, red: dead cells, blue: nuclei, scale bar: 100 μm). (B) CCK-8 assay cell growth profile of GelMA, GelMA@VEGF 165 , and VEGF@bFGF in days 1, 3, 7, 14, and 28 of coculture in complete ADSC and complete M199:ADSC (50:50) medium.

Article Snippet: All experiments were performed with HUVECs between passages 5 to 14. hADSCs were sourced from POIETICS and were cultured in complete ADSC medium (ADSC Growth Media BulletKit, Lonza, Switzerland).

Techniques: Imaging, CCK-8 Assay

Journal: Tissue Engineering. Part C, Methods

Article Title: Ribosomal Protein L13a as a Reference Gene for Human Bone Marrow-Derived Mesenchymal Stromal Cells During Expansion, Adipo-, Chondro-, and Osteogenesis

doi: 10.1089/ten.tec.2012.0081

Figure Lengend Snippet: Distribution of Cq values. (A) Adipo-, (B) chondro-, (C) osteogenesis. Left, values of all donors/passages in control and differentiation culture. Right, data split up into control (grey) and differentiation (black) cultures. (D) All lineages together. (E) RPL13a values of freshly isolated (hBM-MSCs), commercial bone marrow- (Lonza MSCs) and placenta-derived MSCs (hPD-MSCs) as well as HBCs and MG-63. Cq, quantification cycle values; EF1α, eukaryotic translational elongation factor 1 alpha; B2M, β2-microglobulin; ACTB, beta actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; hBM-MSCs, human bone marrow-derived MSCs; hPD-MSCs, human placenta-derived MSCs; HBCs, human bone cells; MG-63, human osteosarcoma cell line.

Article Snippet: The differentiation experiments using Lonza MSCs were performed with purchased cells from one donor and the hPD-MSCs cells from two donors.

Techniques: Isolation, Derivative Assay, Activation Assay